This kit is manufactured and sold under patent license agreement with Shinshu University and Hitachi Solutions, Ltd.
TBONE EX KIT is widely used by Japanese forensic
laboratories in police force and universities.
laboratories in police force and universities.
1. Product features
- Suitable for teeth and bones, genomic DNA of which are deemed difficult to extract.
- No need to grind samples into powder, hence minimize the risk of cross-contamination.
- Extracted DNA can be used for mitochondrial DNA sequencing, genotyping of STR and Y-STR, etc.
2. Procedure of DNA extraction
3. Kit contents
| １ | Solution A | 20 tubes |
| ２ | Solution B | 20 tubes |
| ３ | Solution C | 20 tubes |
| ４ | Protocol | 1 booklet |
4. Reagents to be purchased separately (not included in the kit)
１．DNA Extraction Kit
During DNA extraction procedure, you will need a part of conventional DNA extraction kit.
The followings are examples of DNA extraction kit, which are compatible with TBONE EX KIT.
Please consult with us if you wish to use any alternative method.
| Product | Manufacturer |
|---|---|
| QIAamp® DNA Blood Mini Kit | QIAGEN |
| EZ1 DNA Investigator Kit | QIAGEN |
| PrepFiler® BTA Forensic DNA Extraction Kit | Thermo Fisher Scientific |
２．Equipment
- Hair dryer (optional)
- Incubator
- Centrifuge
３．Other Reagents
- Proteinase K
- Phenol, TE-saturated
- Ethanol Anhydride (for sample cleansing)
４．Consumables
- Neutral detergent (for sample cleansing)
- Tooth brush (for sample cleansing)
- Microcentrifuge tubes
5. Sales information
| Product | Size | Catalog# | Price （Tax not included） |
|---|---|---|---|
| TBONE EX KIT | 20 Preps | TBEW200 | 59,000 JPY / kit |
* excl.tax(8%), shipping cost
6. Reference
- EVALUATION OF A NEW EXPERIMENTAL KIT FOR THE EXTRACTION OF DNA FROM BONES AND TEETH USING A NON-POWDER METHOD.
- Kitayama et.al. Leg Med (Tokyo). 2010 Mar;12(2):84-9. Epub 2010 Jan 27.
- DNA IDENTIFICATION OF SKELETAL REMAINS BY USING A NEW EXTRACTION KIT
- Kazuho Maeda et.al. Forensic Science International: Genetics Supplement Series 4 (2013) e242-e243
- DEVELOPMENT OF A MODIFIED DNA CHIP FOR ACCURATE HUMAN ABO GENOTYPING
- Ken Watanabe et.al. Japanese Journal of Forensic Science and Technology, 20(1), 41-50(2015)
- Presentations at 7th Asian Forensic Sciences Network Annual meeting and Symposium
- Yuko Takagi et.al. Kuala Lumpur. 2015
- Presentation at The Australian and New Zealand Forensic Science Society 23rd International Symposium
- Kageyasu Takanashi et al., Auckland, 2016
7. FAQ
- Q1: Is TBONE EX KIT available for RNA extraction?
- A: No, only DNA can be extracted by this KIT.
- Q2: Can I use TBONE EX KIT to extract samples from animals?
- A: To Apply TBONE EX KIT to animals’ samples is out of warranty.
- Q3: Which part should I pick from bone sample?
- A: Any part can be used.
- Q4: Which kit should I use to purify DNA?
- A: We recommend QIAamp DNA Blood Mini Kit and QIAamp DNA Investigator Kit. PrepFiler is also compatible according to user’s feedback.
- Q5: Is the yield of DNA better if I grind the sample?
- A: It depends on sample condition. Yield of DNA has been reported to be better according to Kitayama et al. 2010, Legal Medicine 12, 84-89.
- Q6: Can I use TBONE EX KIT for extraction from damaged bone sample?
- A: Yes, according to reference, DNA of sample over 60 years had been reported to be successfully extracted.
- Q7: Can I use TBONE EX KIT for extraction from damaged tooth sample?
- A: Yes, according to the reference, DNA of tooth sample over 20 years had been reported successfully extracted.
8. Terms of Use
- This kit is intended for research use only. It cannot be used for purposes other than DNA extraction, such as disease treatment or diagnosis.
- We are not able to provide technical support for trouble shooting or data interpretation if you used methods outside of our provided protocol.
- Please contact each manufacturers if you have questions about reagents and consumables that need to be purchased separately.
- The specification of the kit may be changed without advanced notice.
- This kit is manufactured and sold under patent license agreement with Shinshu University and Hitachi Solutions, Ltd.
- Information last updated: May 2015.
8. Contact Us
- Address :
- 1-15-1 Kaigan, Suzue Baydium 5F
Minato-ku, Tokyo 105-0022, Japan.
- TEL :
- +81 3-5777-1685
- E-mail :
- Web E-mail
- URL :
- http://www.dna-chip.co.jp/
